ckd 581 medchemexpress cat Search Results


94
MedChemExpress c11 bodipy 581 591
A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe <t>BODIPY</t> <t>581/591</t> <t>C11</t> for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.
C11 Bodipy 581 591, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
MedChemExpress ckd 581 medchemexpress cat
A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe <t>BODIPY</t> <t>581/591</t> <t>C11</t> for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.
Ckd 581 Medchemexpress Cat, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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93
MedChemExpress d1148 c11 bodipy 581 591 abclone cat
A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe <t>BODIPY</t> <t>581/591</t> <t>C11</t> for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.
D1148 C11 Bodipy 581 591 Abclone Cat, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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96
Selleck Chemicals erastin
A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe <t>BODIPY</t> <t>581/591</t> <t>C11</t> for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.
Erastin, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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96
Selleck Chemicals plx4032
A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe <t>BODIPY</t> <t>581/591</t> <t>C11</t> for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.
Plx4032, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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94
Selleck Chemicals hy 101193 as1842856 medchemexpress
A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe <t>BODIPY</t> <t>581/591</t> <t>C11</t> for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.
Hy 101193 As1842856 Medchemexpress, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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96
Dojindo Labs mito ferrogreen dojindo cat
A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe <t>BODIPY</t> <t>581/591</t> <t>C11</t> for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.
Mito Ferrogreen Dojindo Cat, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Selleck Chemicals rsl3 medchemexpress cat
A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe <t>BODIPY</t> <t>581/591</t> <t>C11</t> for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.
Rsl3 Medchemexpress Cat, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ckd+581+medchemexpress+cat/RSL3/pm41005298-508-50-45
Average 96 stars, based on 1 article reviews
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95
Selleck Chemicals ike selleck cat
A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe <t>BODIPY</t> <t>581/591</t> <t>C11</t> for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.
Ike Selleck Cat, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Selleck Chemicals 16658b n acetylcysteine medchemexpress cat
A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe <t>BODIPY</t> <t>581/591</t> <t>C11</t> for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.
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16658b n acetylcysteine medchemexpress cat - by Bioz Stars, 2026-11
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96
Selleck Chemicals medchemexpress cat
A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe <t>BODIPY</t> <t>581/591</t> <t>C11</t> for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.
Medchemexpress Cat, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
medchemexpress cat - by Bioz Stars, 2026-11
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A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe BODIPY 581/591 C11 for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.

Journal: Cell Death Discovery

Article Title: Rottlerin triggers dual degradation of SLC7A11 and GPX4 to drive ferroptosis and chemosensitization in hepatocellular carcinoma

doi: 10.1038/s41420-026-02942-1

Figure Lengend Snippet: A HLE and LM3 cells were treated with 2 μM rottlerin or in combination with 2 μM Fer-1. The cells were stained with crystal violet and then dissolved with 10% glacial acetic acid. The absorbance at 595 nm was measured and calculated. Data were represented as mean ± SD ( n = 3) and analyzed using Two-way ANOVA, *** P < 0.001. B LM3 cells were treated with 0.5 μM rottlerin with or without 2 μM Fer-1 for 14 days and then treated with crystal violet stain and photographed. Scale bar = 1 cm. C HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h and then imaged. Scale bar = 100 μm. D HLE and LM3 cells were exposed to the indicated doses of rottlerin with or without 2 μM Fer-1 for 48 h. Cell viability was assessed using the CCK-8 assay. Data were represented as mean ± SD ( n = 3) and analyzed using two-way ANOVA, *** P < 0.001. E HLE and LM3 cells were treated with 5 μM rottlerin with or without 2 μM Fer-1 for 72 h, then stained with PI for flow cytometric analysis. The representative images (top) and cell death rates (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test; ns means not significant, *** P < 0.001. F HLE and LM3 cells were treated with 5 μM rottlerin for 60 h, then stained with the lipid ROS probe BODIPY 581/591 C11 for flow cytometric analysis. The representative images (top) and relative lipid ROS level (bottom) were displayed. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001. G The morphological changes of mitochondria were detected by transmission electron microscopy (TEM) in HLE cells incubated with or without 5 μM rottlerin for 48 h. Scale bar, 500 nm. H Measurement of malondialdehyde (MDA) levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 4) and analyzed using Student’s t -test, * P < 0.05. I Measurement of GSH levels in HLE cells treated with 5 μM rottlerin for 48 h. Data were represented as mean ± SD ( n = 3) and analyzed using Student’s t -test, *** P < 0.001.

Article Snippet: The reagents used in this study are as follows: Rottlerin (TargetMol, Cat# T16791 , USA); RSL3 (CSNpharm, Cat# CSN17581 , USA); Sorafenib (Selleck, Cat# S7397, USA); Ferrostatin-1 (Fer-1, TargetMol, Cat# T6500, USA); C11−BODIPY 581/591 (MCE, Cat# HY-D1691, China); Propidium Iodide (PI, TargetMol, Cat# T2130, USA); 0.25% trypsin digestion solution (Servicebio, Cat# G4012, China); Dimethyl sulfoxide (DMSO, Solarbio, Cat# D8371, China); Cell lysis buffer (CST, Cat# 9803S, USA), PMSF (Wuhan Dingguo Biotechnology, Cat# 329-98-6, China); MG132 (Biovision, Cat# 1791-5, USA); Chloroquine (CQ, MCE, Cat# HY-17589A, China); Cycloheximide (CHX, MCE, Cat# HY-12320, China); Paraformaldehyde (Solarbio, Cat# P1110, China); Sodium dodecyl sulfate (SDS, Solarbio, Cat# S8010, China).

Techniques: Staining, CCK-8 Assay, Transmission Assay, Electron Microscopy, Incubation